Journal of Multidisciplinary Applied Natural Science
Periódico de Acesso Aberto
Scopus CiteScore 2025
2.1
Calculated on 05 May, 2025
SJR 2025
0.25
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Periódico de Acesso Aberto
2.1
Calculated on 05 May, 2025
0.25
Powered by scimagojr.com
https://doi.org/10.47352/jmans.2774-3047.445
Widyastuti Widyastuti
Fendi Setiawan
Ety Apriliana
Wawan A Setiawan
Peni Ahmadi
The growing impact of antimicrobial resistance (AMR) and cancer highlights the need to develop new and more effective therapeutic agents. Here, we explore a solid-state fermentation (SSF) strategy using shrimp-shell waste to stimulate secondary metabolite of marine Kocuria palustris 19C38A1. Bioautography-guided screening revealed that polar components of the extract (C38FA) showed antibacterial activity against multidrug-resistant Staphylococcus aureus (MIC = 250 µg/mL), The same fraction demonstrated pronounced cytotoxicity, inhibiting cell viability of A549 and HeLa cancer cells by 89% and 98%, respectively, at 100 μg mL-1 concentration, while showing weaker activity toward MCF-7 cells. Dereplication analysis using LC–MS/MS has annotated six putative metabolites such as terpendole B (1), p-hydroxyphenylacetylglutamic acid (2), istamycin C1 (3), lankacidin C (4), anthelmycin (5), and octacosa-hexaenoic acid (6) with mass accuracies within ±0.3 ppm. Notably, four of these compounds have well established antibacterial or cytotoxic properties, consistent with the dual in vitro bioactivity observed. ADME predictions suggested that compounds 1 and 2 are the most promising drug-like candidates, showing high gastrointestinal absorption and low cytochrome P450 liability. Furthermore, computational target prediction indicated potential interactions with proteases, kinases, oxidoreductases, and EGFR associated pathways, further hinting at their dual activity multifunctionality. Molecular docking suggested that compound 1 binds to FtsZ and EGFR, with predicted binding energies of -6.89 and -9.03 kcal/mol, respectively. Collectively, this work suggests that shrimp-shell waste can serve as a sustainable biogenic elicitor that induces marine actinobacteria to produce metabolites with dual pharmacological activities under SSF. These findings highlight a sustainable method for discovering potential dual activity antibacterial and anticancer bioactive compounds with therapeutic potential.
[1] I. Gajic, N. Tomic, B. Lukovic, M. Jovicevic, D. Kekic, M. Petrovic, M. Jankovic, A. Trudic, D. M. Culafic, M. Milenkovic, and N. Opavski. (2025). "A Comprehensive Overview of Antibacterial Agents for Combating Multidrug-Resistant Bacteria: The Current Landscape, Development, Future Opportunities, and Challenges". Antibiotics. 14 (3): 221. 10.3390/antibiotics14030221.
DOI: https://doi.org/10.3390/antibiotics14030221[2] K. Ruzindana and R. I. Anorlu. (2025). "Global Disparities in Gynecologic Cancer Outcomes: A Call for Action". International Journal of Gynecology and Obstetrics. 171 (S1): 210-220. 10.1002/ijgo.70278.
DOI: https://doi.org/10.1002/ijgo.70278[3] A. Abdulhak, H. H. Zedan, H. A. El-Mahallawy, A. A. Sayed, H. O. Mohamed, and M. M. Zafer. (2025). "Multidrug-Resistant Pseudomonas Aeruginosa in Immunocompromised Cancer Patients: Epidemiology, Antimicrobial Resistance, and Virulence Factors". BMC Infectious Diseases. 25 (1): 804. 10.1186/s12879-025-11182-0.
DOI: https://doi.org/10.1186/s12879-025-11182-0[4] R. Zhang, S. F. Tan, Y. Wang, J. Wu, and C. Zhang. (2025). "From Macrophage Polarization to Clinical Translation: Immunomodulatory Hydrogels for Infection-Associated Bone Regeneration". Frontiers in Cell and Developmental Biology. 13. 10.3389/fcell.2025.1684357.
DOI: https://doi.org/10.3389/fcell.2025.1684357[5] M. P. Narsing Rao, S. R. Quadri, M. Sathish, N. T. Quach, W. J. Li, and A. Thamchaipenet. (2025). "Exploring Omics Strategies for Drug Discovery from Actinomycetota Isolated from the Marine Ecosystem". Frontiers in Pharmacology. 16. 10.3389/fphar.2025.1634207.
DOI: https://doi.org/10.3389/fphar.2025.1634207[6] L. T. Tan. (2023). "Impact of Marine Chemical Ecology Research on the Discovery and Development of New Pharmaceuticals". Marine Drugs. 21 (3): 174. 10.3390/md21030174.
DOI: https://doi.org/10.3390/md21030174[7] R. Fu, Y. Sun, W. Sheng, and D. Liao. (2017). "Designing Multi-Targeted Agents: An Emerging Anticancer Drug Discovery Paradigm". European Journal of Medicinal Chemistry. 136 : 195-211. 10.1016/j.ejmech.2017.05.016.
DOI: https://doi.org/10.1016/j.ejmech.2017.05.016[8] A. Narayanankutty, A. C. Famurewa, and E. Oprea. (2024). "Natural Bioactive Compounds and Human Health". Molecules. 29 (14): 3372. 10.3390/molecules29143372.
DOI: https://doi.org/10.3390/molecules29143372[9] N. Bano, S. Parveen, M. Saeed, S. Siddiqui, M. Abohassan, and S. S. Mir. (2024). "Drug Repurposing of Selected Antibiotics: An Emerging Approach in Cancer Drug Discovery". ACS Omega. 9 (25): 26762-26779. 10.1021/acsomega.4c00617.
DOI: https://doi.org/10.1021/acsomega.4c00617[10] C. Pfab, L. Schnobrich, S. Eldnasoury, A. Gessner, and N. El-Najjar. (2021). "Repurposing of Antimicrobial Agents for Cancer Therapy: What Do We Know?". Cancers. 13 (13): 3193. 10.3390/cancers13133193.
DOI: https://doi.org/10.3390/cancers13133193[11] L. Fang, L. Xu, M. Kader, T. Ding, S. Lu, D. Wang, A. R. Sharma, and Z. Zhang. (2025). "Salt-Adapted Microorganisms: A Promising Resource for Novel Anti-Cancer Drug Discovery". Marine Drugs. 23 (8): 296. 10.3390/md23080296.
DOI: https://doi.org/10.3390/md23080296[12] A. J. Rathinam, H. Santhaseelan, H. U. Dahms, V. T. Dinakaran, and S. G. Murugaiah. (2023). "Bioprospecting of Unexplored Halophilic Actinobacteria Against Human Infectious Pathogens". 3 Biotech. 13 (12): 398. 10.1007/s13205-023-03812-8.
DOI: https://doi.org/10.1007/s13205-023-03812-8[13] S. Z. Alshawwa, K. S. Alshallash, A. Ghareeb, A. M. Elazzazy, M. Sharaf, A. Alharthi, F. E. Abdelgawad, D. El-Hossary, M. Jaremko, A. Emwas, and Y. A. Helmy. (2022). "Assessment of Pharmacological Potential of Novel Exopolysaccharide Isolated from Marine Kocuria sp. Strain AG5: Broad-Spectrum Biological Investigations". Life. 12 (9): 1387. 10.3390/life12091387.
DOI: https://doi.org/10.3390/life12091387[14] J. Jia, X. Wang, J. Sang, Z. Li, S. Lin, Z. Deng, and T. Huang. (2023). "An N-N Linked Dimeric Indole Alkaloid from the Marine Sponge-Associated Rare Actinomycetes Kocuria sp. S42". Natural Product Research. 37 (21): 3647-3653. 10.1080/14786419.2022.2098496.
DOI: https://doi.org/10.1080/14786419.2022.2098496[15] P. Foti, C. Caggia, and F. V. Romeo. (2025). "New Insight into Microbial Exploitation to Produce Bioactive Molecules from Agrifood and By-Products' Fermentation". Foods. 14 (8). 10.3390/foods14081439.
DOI: https://doi.org/10.3390/foods14081439[16] Z. Wang, D. Zeng, Y. Zhu, M. Zhou, A. Kondo, T. Hasunuma, and X. Zhao. (2025). "Fermentation Design and Process Optimization Strategy Based on Machine Learning". BioDesign Research. 7 (1): 100002. 10.1016/j.bidere.2025.100002.
DOI: https://doi.org/10.1016/j.bidere.2025.100002[17] A. Setiawan, W. Widyastuti, A. Irawan, O. S. Wijaya, A. Laila, W. A. Setiawan, N. L. G. R. Juliasih, K. Nonaka, M. Arai, and J. Hendri. (2021). "Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites". Fermentation. 7 (4): 247. 10.3390/fermentation7040247.
DOI: https://doi.org/10.3390/fermentation7040247[18] X. Yang, L. Yuan, M. Zeeshan, C. Yang, W. Gao, G. Zhang, and C. Wang. (2025). "Optimization of Fermentation Conditions to Increase the Production of Antifungal Metabolites from Streptomyces sp. KN37". Microbial Cell Factories. 24 (1): 26. 10.1186/s12934-025-02652-w.
DOI: https://doi.org/10.1186/s12934-025-02652-w[19] M. Suliman, A. S. Bishr, S. T. K. Tohamy, M. Y. Alshahrani, and K. M. Aboshanab. (2025). "Solid-State Fermentation of Pristinamycin by Streptomyces pristinaespiralis NRRL ISP-5338 Using D-Optimal Design". Bioprocess and Biosystems Engineering. 48 (9): 1467-1479. 10.1007/s00449-025-03188-4.
DOI: https://doi.org/10.1007/s00449-025-03188-4[20] X. Ma, G. Gözaydın, H. Yang, W. Ning, X. Han, N. Y. Poon, H. Liang, N. Yan, and K. Zhou. (2020). "Upcycling Chitin-Containing Waste into Organonitrogen Chemicals via an Integrated Process". Proceedings of the National Academy of Sciences of the United States of America. 117 (14): 7719-7728. 10.1073/pnas.1919862117.
DOI: https://doi.org/10.1073/pnas.1919862117[21] A. Setiawan, F. Setiawan, N. L. G. R. Juliasih, W. Widyastuti, A. Laila, W. A. Setiawan, F. M. Djailani, M. Mulyono, J. Hendri, and M. Arai. (2022). "Fungicide Activity of Culture Extract from Kocuria palustris 19C38A1 Against Fusarium oxysporum". Journal of Fungi. 8 (3): 280. 10.3390/jof8030280.
DOI: https://doi.org/10.3390/jof8030280[22] R. Subramani and W. Aalbersberg. (2013). "Culturable Rare Actinomycetes: Diversity, Isolation and Marine Natural Product Discovery". Applied Microbiology and Biotechnology. 97 (21): 9291-9321. 10.1007/s00253-013-5229-7.
DOI: https://doi.org/10.1007/s00253-013-5229-7[23] W. Widyastuti, F. Setiawan, C. A. Afandy, A. Irawan, A. Laila, N. L. G. R. Juliasih, W. A. Setiawan, M. Arai, J. Hendri, and A. Setiawan. (2022). "Antifungal Agent Chitooligosaccharides Derived from Solid-State Fermentation of Shrimp Shell Waste by Pseudonocardia antitumoralis 18D36-A1". Fermentation. 8 (8): 353. 10.3390/fermentation8080353.
DOI: https://doi.org/10.3390/fermentation8080353[24] A. Setiawan, F. Setiawan, S. Susianti, W. A. Setiawan, P. Ahmadi, R. Pangestu, J. Hendri, and N. L. G. R. Juliasih. (2025). "Chemical Profile of The Ethyl Acetate Extract of Aspergillus sydowi 22-PLP1-F1 as Antibacterial Agent Against Clinically Resistant Strains of Staphylococcus aureus and Pseudomonas aeruginosa". Journal of Multidisciplinary Applied Natural Science. 5 (1). 10.47352/jmans.2774-3047.238.
DOI: https://doi.org/10.47352/jmans.2774-3047.238[25] A. Setiawan, R. Lutfiah, N. L. G. R. Juliasih, W. A. Setiawan, J. Hendri, and M. Arai. (2022). "Antibacterial Activity of EtOAc Extract from Marine-Derived Fungus Aspergillus nomiae A12-RF Against Clinical Pathogen Bacteria, Staphylococcus aureus". AACL Bioflux. 15 (3): 1413-1421.
[26] P. Ahmadi, M. Higashi, N. J. D. Voogd, and J. Tanaka. (2017). "Two Furanosesterterpenoids from the Sponge Luffariella variabilis". Marine Drugs. 15 (8): 249. 10.3390/md15080249.
DOI: https://doi.org/10.3390/md15080249[27] R. Schmid, S. Heuckeroth, A. Korf, A. Smirnov, O. Myers, T. S. Dyrlund, R. Bushuiev, K. J. Murray, N. Hoffmann, M. Lu, A. Sarvepalli, Z. Zhang, M. Fleischauer, K. Dührkop, M. Wesner, S. J. Hoogstra, E. Rudt, O. Mokshyna, and C. Brungs. (2023). "Integrative Analysis of Multimodal Mass Spectrometry Data in MZmine 3". Nature Biotechnology. 41 (4): 447-449. 10.1038/s41587-023-01690-2.
DOI: https://doi.org/10.1038/s41587-023-01690-2[28] A. Daina, O. Michielin, and V. Zoete. (2017). "SwissADME: A Free Web Tool to Evaluate Pharmacokinetics, Drug-Likeness and Medicinal Chemistry Friendliness of Small Molecules". Scientific Reports. 7 (1): 42717. 10.1038/srep42717.
DOI: https://doi.org/10.1038/srep42717[29] A. Daina and V. Zoete. (2024). "Testing the Predictive Power of Reverse Screening to Infer Drug Targets, with the Help of Machine Learning". Communications Chemistry. 7 (1): 105. 10.1038/s42004-024-01179-2.
DOI: https://doi.org/10.1038/s42004-024-01179-2[30] E. F. Pettersen, T. D. Goddard, C. C. Huang, G. S. Couch, D. M. Greenblatt, E. C. Meng, and T. E. Ferrin. (2004). "UCSF Chimera: A Visualization System for Exploratory Research and Analysis". Journal of Computational Chemistry. 25 (13): 1605-1612. 10.1002/jcc.20084.
DOI: https://doi.org/10.1002/jcc.20084[31] I. V. Ferrari and P. Patrizio. (2021). "Development and Validation Molecular Docking Analysis of Human Serum Albumin (HSA)". bioRxiv. 10.1101/2021.07.09.451789.
DOI: https://doi.org/10.1101/2021.07.09.451789[32] J. Fuhrmann, A. Rurainski, H. P. Lenhof, and D. Neumann. (2010). "A New Lamarckian Genetic Algorithm for Flexible Ligand-Receptor Docking". Journal of Computational Chemistry. 31 (9): 1911-1918. 10.1002/jcc.21478.
DOI: https://doi.org/10.1002/jcc.21478[33] N. K. Subramani and S. Venugopal. (2024). "Molecular Docking and Dynamic Simulation Studies of Bioactive Compounds from Traditional Medicinal Compounds Against Exfoliative Toxin B from Staphylococcus aureus". Journal of Pharmacology and Pharmacotherapeutics. 15 (3): 316-326. 10.1177/0976500X241266072.
DOI: https://doi.org/10.1177/0976500X241266072[34] A. van der Meij, H. Tyrrell, D. J. Sokolowski, E. M. F. Shepherdson, M. A. Elliot, and J. R. Nodwell. (2025). "Streptomyces venezuelae Uses Secreted Chitinases and a Designated ABC Transporter to Support the Competitive Saprophytic Catabolism of Chitin". PLOS Biology. 23 (8): e3003292. 10.1371/journal.pbio.3003292.
DOI: https://doi.org/10.1371/journal.pbio.3003292[35] X. Sun, Y. Zhao, and G. Ding. (2023). "Morphogenesis and Metabolomics Reveal the Compatible Relationship Among Suillus bovinus, Phialocephala fortinii, and Their Co-Host, Pinus massoniana". Microbiology Spectrum. 11 (5): e01453-23. 10.1128/spectrum.01453-23.
DOI: https://doi.org/10.1128/spectrum.01453-23[36] C. Chakansin, J. Yostaworakul, C. Warin, K. Kulthong, and S. Boonrungsiman. (2022). "Resazurin Rapid Screening for Antibacterial Activities of Organic and Inorganic Nanoparticles: Potential, Limitations and Precautions". Analytical Biochemistry. 637 : 114449. 10.1016/j.ab.2021.114449.
DOI: https://doi.org/10.1016/j.ab.2021.114449[37] A. M. Mayer, M. L. Pierce, K. Howe, A. D. Rodríguez, O. Taglialatela-Scafati, F. Nakamura, and N. Fusetani. (2022). "Marine Pharmacology in 2018: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action". Pharmacological Research. 183 : 106391. 10.1016/j.phrs.2022.106391.
DOI: https://doi.org/10.1016/j.phrs.2022.106391[38] A. T. Dharmaraja. (2017). "Role of Reactive Oxygen Species (ROS) in Therapeutics and Drug Resistance in Cancer and Bacteria". Journal of Medicinal Chemistry. 60 (8): 3221-3240. 10.1021/acs.jmedchem.6b01243.
DOI: https://doi.org/10.1021/acs.jmedchem.6b01243[39] A. González, D. Vázquez, and A. Jiménez. (1979). "Inhibition of Translation in Bacterial and Eukaryotic Systems by the Antibiotic Anthelmycin (Hikizimycin)". Biochimica et Biophysica Acta - Nucleic Acids and Protein Synthesis. 561 (2): 403-409. 10.1016/0005-2787(79)90148-5.
DOI: https://doi.org/10.1016/0005-2787(79)90148-5[40] A. Hirata, M. Sumiyoshi, H. Fujita, M. Akimoto, M. H. R. A. Padayao, Y. Eguchi, M. Matsuura, M. Otsuka, K. Inada, A. Teshima, and K. Arakawa. (2025). "Rare Distribution of Butenolide-Type Signaling Molecules Among Streptomyces Strains and Functional Importance as Inducing Factors for Secondary Metabolite Production in Streptomyces rochei 7434AN4". The Journal of Antibiotics. 78 (8): 488-499. 10.1038/s41429-025-00840-9.
DOI: https://doi.org/10.1038/s41429-025-00840-9[41] Y. Hongo, T. Nakamura, S. Takahashi, T. Motoyama, T. Hayashi, H. Hirota, H. Osada, and H. Koshino. (2014). "Detection of Oxygen Addition Peaks for Terpendole E and Related Indole-Diterpene Alkaloids in a Positive-Mode ESI-MS". Journal of Mass Spectrometry. 49 (6): 537-542. 10.1002/jms.3360.
DOI: https://doi.org/10.1002/jms.3360[42] S. Rai, L. S. Singh, K. Liriina, K. Jeyaram, T. Parija, and D. Sahoo. (2025). "Novel Endophytic Actinomycetes Species Streptomyces panacea of Panax sokpayensis Produce Antimicrobial Compounds Against Multidrug Resistant Staphylococcus aureus". Scientific Reports. 15 (1): 19863. 10.1038/s41598-025-05333-1.
DOI: https://doi.org/10.1038/s41598-025-05333-1[43] F. A. M. Gomaa, H. M. R. M. Selim, M. Y. Alshahrani, and K. M. Aboshanab. (2024). "Central Composite Design for Optimizing Istamycin Production by Streptomyces tenjimariensis". World Journal of Microbiology and Biotechnology. 40 (10): 316. 10.1007/s11274-024-04118-4.
DOI: https://doi.org/10.1007/s11274-024-04118-4[44] S. Demisie, D. Oh, A. Abera, G. Tasew, G. D. Satessa, F. Fufa, A. M. Shenkutie, D. Wolday, and K. Tafess. (2024). "Bioprospecting Secondary Metabolites with Antimicrobial Properties from Soil Bacteria in High-Temperature Ecosystems". Microbial Cell Factories. 23 (1): 332. 10.1186/s12934-024-02589-6.
DOI: https://doi.org/10.1186/s12934-024-02589-6[45] Y. Hou, M. Chen, Z. Sun, G. Ma, D. Chen, H. Wu, J. Yang, Y. Li, and X. Xu. (2022). "The Biosynthesis Related Enzyme, Structure Diversity and Bioactivity Abundance of Indole-Diterpenes: A Review". Molecules. 27 (20): 6870. 10.3390/molecules27206870.
DOI: https://doi.org/10.3390/molecules27206870[46] C. A. Lipinski. (2016). "Rule of Five in 2015 and Beyond: Target and Ligand Structural Limitations, Ligand Chemistry Structure and Drug Discovery Project Decisions". Advanced Drug Delivery Reviews. 101 : 34-41. 10.1016/j.addr.2016.04.029.
DOI: https://doi.org/10.1016/j.addr.2016.04.029[47] S. P. Sweety, T. A. Rupok, S. A. Shoily, S. Parvin, J. Barmon, and M. E. Islam. (2025). "GC-MS Analysis, Molecular Docking and Pharmacokinetic Evaluation of Phytocompounds from Lagerstroemia speciosa (L.) Pers. Bark and Their Effect on Inflammation Target Proteins". Food Chemistry Advances. 9 : 101172. 10.1016/j.focha.2025.101172.
DOI: https://doi.org/10.1016/j.focha.2025.101172[48] D. Garcia Jimenez, V. Poongavanam, and J. Kihlberg. (2023). "Macrocycles in Drug Discovery—Learning from the Past for the Future". Journal of Medicinal Chemistry. 66 (8): 5377-5396. 10.1021/acs.jmedchem.3c00134.
DOI: https://doi.org/10.1021/acs.jmedchem.3c00134[49] F. Susa, S. Arpicco, C. F. Pirri, and T. Limongi. (2024). "An Overview on the Physiopathology of the Blood-Brain Barrier and the Lipid-Based Nanocarriers for Central Nervous System Delivery". Pharmaceutics. 16 (7): 849. 10.3390/pharmaceutics16070849.
DOI: https://doi.org/10.3390/pharmaceutics16070849[50] A. Talevi and C. L. Bellera. (2022). In: "The ADME Encyclopedia". Cham: Springer Nature Switzerland. 10.1007/978-3-030-84860-6_73.
DOI: https://doi.org/10.1007/978-3-030-84860-6_73[51] S. Kalyaanamoorthy, S. M. Lamothe, X. Hou, T. C. Moon, H. T. Kurata, M. Houghton, and K. H. Barakat. (2020). "A Structure-Based Computational Workflow to Predict Liability and Binding Modes of Small Molecules to hERG". Scientific Reports. 10 (1): 16262. 10.1038/s41598-020-72889-5.
DOI: https://doi.org/10.1038/s41598-020-72889-5[52] A. Claesson and A. Minidis. (2018). "Systematic Approach to Organizing Structural Alerts for Reactive Metabolite Formation from Potential Drugs". Chemical Research in Toxicology. 31 (6): 389-411. 10.1021/acs.chemrestox.8b00046.
DOI: https://doi.org/10.1021/acs.chemrestox.8b00046[53] K. C. Baral and K. Y. Choi. (2025). "Barriers and Strategies for Oral Peptide and Protein Therapeutics Delivery: Update on Clinical Advances". Pharmaceutics. 17 (4): 397. 10.3390/pharmaceutics17040397.
DOI: https://doi.org/10.3390/pharmaceutics17040397[54] S. A. Putri, R. Maharani, I. P. Maksum, and T. J. Siahaan. (2025). "Peptide Design for Enhanced Anti-Melanogenesis: Optimizing Molecular Weight, Polarity, and Cyclization". Drug Design, Development and Therapy. 19 : 645-670. 10.2147/DDDT.S500004.
DOI: https://doi.org/10.2147/DDDT.S500004[55] L. Ali and M. H. Abdel Aziz. (2024). "Crosstalk Involving Two-Component Systems in Staphylococcus aureus Signaling Networks". Journal of Bacteriology. 206 (4): e00418-23. 10.1128/jb.00418-23.
DOI: https://doi.org/10.1128/jb.00418-23[56] M. Wang, C. Fang, B. Ma, X. Luo, and Z. Hou. (2020). "Regulation of Cytokinesis: FtsZ and Its Accessory Proteins". Current Genetics. 66 (1): 43-49. 10.1007/s00294-019-01005-6.
DOI: https://doi.org/10.1007/s00294-019-01005-6[57] S. C. Gupta, S. Prasad, J. H. Kim, S. Patchva, L. J. Webb, I. K. Priyadarsini, and B. B. Aggarwal. (2011). "Multitargeting by Curcumin as Revealed by Molecular Interaction Studies". Natural Product Reports. 28 (12): 1937-1955. 10.1039/C1NP00051A.
DOI: https://doi.org/10.1039/c1np00051a[58] Y. Zheng, R. Du, S. Cai, Z. Liu, Z. Fang, T. Liu, L. So, Y. Lu, N. Sun, and K. Wong. (2018). "Study of Benzofuroquinolinium Derivatives as a New Class of Potent Antibacterial Agent and the Mode of Inhibition Targeting FtsZ". Frontiers in Microbiology. 9 : 1937. 10.3389/fmicb.2018.01937.
DOI: https://doi.org/10.3389/fmicb.2018.01937[59] J. M. Barrows and E. D. Goley. (2021). "FtsZ Dynamics in Bacterial Division: What, How, and Why?". Current Opinion in Cell Biology. 68 : 163-172. 10.1016/j.ceb.2020.10.013.
DOI: https://doi.org/10.1016/j.ceb.2020.10.013[60] A. M. Tovar-Nieto, L. E. Flores-Padilla, B. Rivas-Santiago, J. V. Trujillo-Paez, E. E. Lara-Ramirez, Y. M. Jacobo-Delgado, J. E. López-Ramos, and A. Rodríguez-Carlos. (2024). "The Repurposing of FDA-Approved Drugs as FtsZ Inhibitors Against Mycobacterium tuberculosis: An In Silico and In Vitro Study". Microorganisms. 12 (8): 1505. 10.3390/microorganisms12081505.
DOI: https://doi.org/10.3390/microorganisms12081505[61] K. Shi, G. Wang, J. Pei, J. Zhang, J. Wang, L. Ouyang, Y. Wang, and W. Li. (2022). "Emerging Strategies to Overcome Resistance to Third-Generation EGFR Inhibitors". Journal of Hematology and Oncology. 15 (1): 94. 10.1186/s13045-022-01311-6.
DOI: https://doi.org/10.1186/s13045-022-01311-6[62] L. Saldaña-Rivera, M. Bello, and D. Méndez-Luna. (2019). "Structural Insight into the Binding Mechanism of ATP to EGFR and L858R, and T790M and L858R/T790 Mutants". Journal of Biomolecular Structure and Dynamics. 37 (17): 4671-4684. 10.1080/07391102.2018.1558112.
DOI: https://doi.org/10.1080/07391102.2018.1558112[63] M. L. Uribe, I. Marrocco, and Y. Yarden. (2021). "EGFR in Cancer: Signaling Mechanisms, Drugs, and Acquired Resistance". Cancers. 13 (11): 2748. 10.3390/cancers13112748.
DOI: https://doi.org/10.3390/cancers13112748[64] P. N. Sonwane and M. R. Kumbhare. (2025). "Molecular Docking and Pharmacokinetics of Benzimidazole-Based FtsZ Inhibitors for Tuberculosis". Scientific Reports. 15 (1): 35270. 10.1038/s41598-025-18084-w.
DOI: https://doi.org/10.1038/s41598-025-18084-w[65] M. S. R, Y. H. S, S. M. A. Sangi, A. K. N, S. Shaik, S. Nagaraja, G. Meravanige, M. M. Islam, P. K. Sreenivasalu, R. M. Almuqbil, S. Chohan, B. Pandey, S. Thapa, and A. V. Atoki. (2026). "In Silico Screening of Marine Fungal Metabolites Identifies Potential FtsZ Inhibitors Against MDR-Tuberculosis Through Docking and Molecular Dynamics Analysis". Scientific Reports. 16 (1): 4030. 10.1038/s41598-025-34116-x.
DOI: https://doi.org/10.1038/s41598-025-34116-x[66] M. Fan, L. Hu, S. Shi, X. Song, H. He, and B. Qi. (2023). "Design, Synthesis and Biological Evaluation of EGFR Kinase Inhibitors That Span the Orthosteric and Allosteric Sites". Bioorganic and Medicinal Chemistry. 96 : 117534. 10.1016/j.bmc.2023.117534.
DOI: https://doi.org/10.1016/j.bmc.2023.117534